Analysis of SSRs Derived from an EST Library of Half-Smooth Tongue Sole Cynoglossus semilaevis

A complementary DNA (cDNA) library was constructed from half-smooth tongue sole spleen. A long-read expressed sequence tag (EST) database was generated, containing 3100 cDNA clones, of which 220 clones were fully sequenced. A total of 1060 non-redundant simple sequence repeats (SSRs) were obtained from the cDNA library. An average of 5 kb sequence generates 1 SSR in the half-smooth tongue sole spleen cDNA library. The proportion of the SSR unit size was different in the cDNA library. The monomeric repeats (51.4%) are the most abundant class of SSR in the dataset. The dimeric, trimeric, tetrameric and hexameric re- peats are represented in decreasing proportions of 27.2%, 16.0%, 2.8% and 1.9%, respectively. The frequency of pentameric repeats was observed the least (only 0.7%). Most of the monomeric and dimeric repeats are distributed in 3' and 5' un-translation region. If translation regions are considered merely, trimeric repeats are the highest, accounting for 57% of the total microsatellites.

作 者： LIU Yunguo BAO Baolong CHEN Songlin SUN Xiuqin   作者單位： LIU Yunguo(College of Fisheries, Ocean University of China, Qingdao 266003, P. R. China;Key Laboratory of Marine and Estuarine Fisheries, Ministry of Agriculture, East China Sea Fisheries Research Institute, Shanghai 200090, P. R. China)

BAO Baolong(College of Aqua-Lite Science and Technology, Shanghai Ocean University, Shanghai 200090, P. R. China)

CHEN Songlin(Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, P. R. China)

SUN Xiuqin(The First Institute q/Oceanography, State Oceanic Administration, Qingdao 266061, P. R. China) 

刊 名： 中國海洋大學學報（英文版）  ISTIC 英文刊名： JOURNAL OF OCEAN UNIVERSITY OF CHINA  年，卷(期)： 2008 7(3)  分類號： P7  關鍵詞： half-smooth tongue sole   Cynoglossus semilaevis   EST   microsatellite  

【Analysis of SSRs Derived from an EST】相關文章：

A General Method for QTL Mapping in Multiple Related Populations Derived from Multiple Parents04-26

Effect of pyrolysis temperature and hold time on the characteristic parameters of adsorbent derived from sewage sludge04-25

In Silico Cloning and Sequence Analysis of Phospholipase Dα Gene from Peach Fruit04-28

Purification Influence of Synthesis Gas Derived from Methanol Cracking on the Performance of Cobalt Catalyst in Fischer-04-29

Spectral analysis of quasi-stationary sea surface topography from GRACE mission04-29

Bioavailability of bound residue derived from 14 C-labeled chlorsulfuron in soil and its mechanism of phytotoxicity04-26

Low-Temperature Response to Major Agronomic Traits by Using Recombinant Inbred Line (RIL) Populations Derived from Towad04-28

Identification of the bound residue composition derived from 14C-labeled chlorsulfuron in soil by using LC-MS and isotop04-26

Syntheses and Crystal Structures of Two Mononuclear Tin(IV) Complexes Derived from Sn(edt)2 (edt = Ethane-1,2-dithiolate04-26

ORGANO-SOLUBLE FLUORINATED POLYIMIDES DERIVED FROM α,α-BIS(4-AMINO-3,5- DIMETHYLPHENYL)-4-FLUOROPHENYL METHANE AND VARIO04-28